Use of Modern Techniques for Salt Tolerance Studies
Crop improvement strategies based on molecular marker techniques and biotechnology can be used in conjunction with breeding efforts for this purpose. DNA markers enhance the recovery rate of isogenic recurrent genome after hybridization and facilitate introgression of quantitative trait loci (QTL) necessary to increase stress tolerance. Molecular marker techniques were used to transfer alleles of interest from wild relatives into cultivated cultivars.
In comparison with other molecular markers, SSR or microsatellite markers have gained much attention as they have higher levels of polymorphism than other DNA marker systems in many crops. They have been successfully used for mapping, cultivar identification, protecting germplasm, determination of hybridity, and as diagnostic markers for traits of economic value. SARC is working on these biotechnological aspects to tackle the major constraints of the crop production including transformation and QTLs as well as physiological approaches. The main target is to develop plants with higher yields under salt stress.
Gel photograph showing SSR polymorphism for 24 wheat doubled
haploid lines with the SSR primer gwm-249-2A
RAPD- PCR of 20 wheat genotypes with OPC-10 primer.
M=1Kb ladder, 1-20 are wheat genotypes